INVESTIGATION OF SUPERCRITICAL CO2-EXTRACTS OF WILD LEDUM PALUSTRE L. (RHODODENDRON TO-MENTOSUM HARMAJA) AND IDENTIFICATION OF ITS METABOLITES BY TANDEM MASS SPECTROMETRY

The purpose of this research is to investigate and identify polyphenolic complexes other biologically active compounds by tandem mass spectrometry, presented in the leaves stems Ledum palustre L. Carbon dioxide, compressed a supercritical state, was used for most environmentally friendly extraction effective characteristics (pressure 350 bar; temperature 60 °С; time 1-hour, co-solvent MeOH 3.5%) CO2-extraction were obtained empirically. To target analytes extracts, High Performance Liquid Chromatography (HPLC) combination with BRUKER DALTONIKS ion trap. results showed presence 61 corresponding rhododendron species, which 32 identified first palustre. These are flavanols dihydrokaempferol, quercetin arabinoside, myricetin galactoside; flavones: diosmetin, nevadensin, cirsimaritin; flavanone naringenin; anthocyanins delphinidin, petunidin, cyanidin pentoside, delphinidin peonidin 3-(6-O-acetyl) glucoside, peonidin-3-O-malonylglucoside, cyanidin-3-rutinoside, 3-O-glucoside; ellagic acid; lignan medioresinol; type A procyanidin dimer; sterols fucosterol avenasterol, etc.